Cleavable C-terminal His-tag vectors for structure determination
نویسندگان
چکیده
منابع مشابه
Synthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
متن کاملSynthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
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Cytochrome c maturation in the periplasms of many bacteria requires the heme chaperone CcmE, which binds heme covalently both in vivo and in vitro via a histidine residue before transferring the heme to apocytochromes c. To investigate the mechanism and specificity of heme attachment to CcmE, we have mutated the conserved histidine 130 of a soluble C-terminally His-tagged version of CcmE (CcmEs...
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recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...
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In this paper, we focus on the structured multi-frame vectors in Hilbert $C^*$-modules. More precisely, it will be shown that the set of all complete multi-frame vectors for a unitary system can be parameterized by the set of all surjective operators, in the local commutant. Similar results hold for the set of all complete wandering vectors and complete multi-Riesz vectors, when the surjective ...
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ژورنال
عنوان ژورنال: Journal of Structural and Functional Genomics
سال: 2010
ISSN: 1345-711X,1570-0267
DOI: 10.1007/s10969-010-9082-y